INFLUENCE OF DIFFERENT TITANIUM SURFACE TREATMENTS ON THE BIOLOGICAL BEHAVIOR OF OSTEOBLASTIC CELLS

Resumo

Introduction: Different methods of treatment to dental titanium surfaces have been adopted to improve mechanical and chemical properties of the implant and to improve osseous integration. Objective:The objective of the present work was to compare the performance of three titanium surfaces, Porous® (obtained by double acid treatment), Vulcano Actives® (obtained by anodization), and PorousNano® (obtained by double acid treatment and fluoride incorporation) in regards to cell proliferation of osteoblast precursors and gene expression of extracellular matrix proteins type 1 collagen, osteocalcin, osteopontin and ostenectin. Materials and Methods: We utilized commercially pure grade 4 titanium discs to cultivate osteoblastic precursor cell line MC3T3-E1 (ATCC - USA). After 3, 7 and 10 days, we performed cell proliferation assay using Trypan blue exclusion testand gene expression analysis by Real-Time PCR. Results and Conclusion: Results were recorded and analyzed statistically using a level of significance of P<0.05. We observed higher cell proliferation and higher gene expression of proteins linked to synthesis and mineralization of the extracellular matrix on the Vulcano Actives® surface.